11-05-2018, 11:18 AM
Prepare with the help of Model Question Paper for Int-PhD entrance exam held at IIT-JAM/ TIFR-NCBS/ IIT Kgp-IACS / Bose Institute- CU (Module: Advanced bio-physical techniques)
Courtesy: Bio Frontiers
Prepared by: Sumit Mukherjee, Department of Life Science, NIT, Rourkela, India
1.   Fluorescence recovery after photobleaching in live cells is used to determine
(a)Â Â Â Flip-flop movement of membrane lipids
(b)Â Â Â Lateral movement of transmembrane proteins
©Â Â Â Movement of peripheral proteins
(d)Â Â Â Co-localization of proteins
2.   Fluorescence involves the excitation and emission of light based on the theory that
(a)Â Â Â The emission and excitation wavelength arc same
(b)Â Â Â The emission wavelength is shorter than the excitation wavelength
©Â Â Â The emission wavelength is longer than the excitation wavelength
(d)Â Â Â None of the above
3.   FRET stands for
(a)Â Â Â Fluroscence recovery energy transfer
(b)Â Â Â Fluroscence resonance energy transfer
©Â Â Â Fluroscence resonance electron transfer
(d)Â Â Â Fluroscence recovery electron transfer
4.   Three proteins are moving together as a single band in a SDS-PAGE after loading in a single lane. Which of the following technique can be used to identify them?
(a)Â Â Â Mass spectroscopy
(b)Â Â Â NMR
©Â Â Â Native PAGE
(d)Â Â Â Western blot
5.   A protein undergoes a post-translational modification. In order to identify the nature of modification some experiments were performed and the results are as follows:
1.   Protein moved slowly in SDS-PAGE
2.   Isoelectric focusing (1EF) shows that there was no change in pi
3.   Mass spectrometric analysis showed that the modification was on serine
Based on these observations, it is more likely that the modification was a
(a)Â Â Â Phosphorylation
(b)Â Â Â Glycosylation
©Â Â Â ADP-ribosylation
(d)Â Â Â Ubiquitination
Courtesy: Bio Frontiers
Prepared by: Sumit Mukherjee, Department of Life Science, NIT, Rourkela, India
1.   Fluorescence recovery after photobleaching in live cells is used to determine
(a)Â Â Â Flip-flop movement of membrane lipids
(b)Â Â Â Lateral movement of transmembrane proteins
©Â Â Â Movement of peripheral proteins
(d)Â Â Â Co-localization of proteins
2.   Fluorescence involves the excitation and emission of light based on the theory that
(a)Â Â Â The emission and excitation wavelength arc same
(b)Â Â Â The emission wavelength is shorter than the excitation wavelength
©Â Â Â The emission wavelength is longer than the excitation wavelength
(d)Â Â Â None of the above
3.   FRET stands for
(a)Â Â Â Fluroscence recovery energy transfer
(b)Â Â Â Fluroscence resonance energy transfer
©Â Â Â Fluroscence resonance electron transfer
(d)Â Â Â Fluroscence recovery electron transfer
4.   Three proteins are moving together as a single band in a SDS-PAGE after loading in a single lane. Which of the following technique can be used to identify them?
(a)Â Â Â Mass spectroscopy
(b)Â Â Â NMR
©Â Â Â Native PAGE
(d)Â Â Â Western blot
5.   A protein undergoes a post-translational modification. In order to identify the nature of modification some experiments were performed and the results are as follows:
1.   Protein moved slowly in SDS-PAGE
2.   Isoelectric focusing (1EF) shows that there was no change in pi
3.   Mass spectrometric analysis showed that the modification was on serine
Based on these observations, it is more likely that the modification was a
(a)Â Â Â Phosphorylation
(b)Â Â Â Glycosylation
©Â Â Â ADP-ribosylation
(d)Â Â Â Ubiquitination